Native SNARE complexes from insulinoma INS8332/13 lysates were isolated by incubation with anti-VAMP2 or VAMP8 antibody-coated superparamagnetic beads followed by extensive washing, then purified α-SNAP and NSF added in presence of ATP-Mg2+ or EDTA (Fig. 3c). Here, VAMP2 is linked to pancreatic insulinoma.