In vivo, EGF-treated TMZ-R animals displayed a more normalized tumor volume/weight only when EGF was paired with TMZ treatment, but not by EGF alone, suggesting that while EGF can help quiescent CSCs revert to their differentiated state and the associated tumorigenic phenotypes, it cannot act alone as an effective glioblastoma therapeutic. Here, EGF is linked to glioblastoma.