To examine the role of CB1 on in vitro network activity following chronic BCC treatment, we left neurons untreated (naive) or treated with BCC (downscaled) for 40 h, and then monitored changes in spiking activities during 6 h of CB1 inhibition using 50 nm AM251 (Fig. 5A). This evidence concerns the gene CNR1 and skin basal cell carcinoma.