However, in the IFNγ KO mice, although the infection was largely controlled by the prophylaxis treatment, the systemic infection was barely detectable during the prophylactic treatment period (S1 Fig) and within 4 weeks of termination of BNZ prophylaxis, 4 of 5 mice in the previously treated IFNγ KO mice group were systemically parasite-positive and had to be euthanized (Fig 1C and S1 Fig). Here, IFNG is linked to infection.