To study NLRP1 activation in response to viral infection, we thus infected HEKNLRP1+ASC, HEKNLRP3+ASC, and N/TERT-1C1C-EGFP cells with SFV, the closely related Sindbis virus (SINV), as well as vesicular stomatitis virus (VSV), a negative-sense single-stranded RNA virus. Here, NLRP1 is linked to viral infectious disease.