Due to the loss of spatial information with single-cell transcriptomics, protein marker staining with CD163 (for M2-like TAMs), PD-1, and TIM-3 (for exhausted CD8+) has been used to confirm virtual co-localization within the ccRCC microenvironment (43). The gene discussed is HAVCR2; the disease is nonpapillary renal cell carcinoma.