Our research found the affinitive relationship between twenty cuproptosis-related genes expression (FDX1, LIPT1, DLAT, PDHA1, MTF1, MT-CO1, ACO1, SCO2, ATP7A, SLC25A3, AOC1, COA6, TMEM199, ATP6AP1, ACP1, FDX2, NUBP2, CIAPIN1, ISCA2, and NDOR1) and the prognosis of HCC. This evidence concerns the gene ACO1 and hepatocellular carcinoma.