These results suggested that FDX1, LIPT1, DLAT, PDHA1, MTF1, MT-CO1, ACO1, SCO2, ATP7A, SLC25A3, AOC1, COA6, TMEM199, ATP6AP1, ACP1, FDX2, NUBP2, CIAPIN1, ISCA2, and NDOR1 may serve as prognostic markers and potential therapeutic targets in HCC, which were not summarized in other bioinformatic analysis research of HCC. This evidence concerns the gene MT-CO1 and hepatocellular carcinoma.