For example, PD-1 (Programmed cell death protein 1) checkpoint inhibitor can bind to PD-1 or its ligand PD-L1 and block PD-1 from blocking T cell (103).But the current effect of immune checkpoint inhibitors represented by PD-1 in the treatment of glioma is not very satisfactory (104, 105). The gene discussed is PDCD1; the disease is glioma.