Interestingly, similar to ARF−/− MEFs with increased expression of Aurora B, MDA-MB-231 cells stably expressing untagged Aurora B in response to tetracycline, as well as MCF7 breast cancer cells stably expressing Aurora B tagged with the mNeonGreen version of GFP at the C-terminus in response to tetracycline, also exhibited improved growth in response to ZM447439 at both the population and single cell levels (Figures 4L–O). This evidence concerns the gene CDKN2A and breast carcinoma.