When comparing average gene expression of cell type signature genes between VAT and SAT (Figure 2E), we identified several DEGs that were depot-specific, such as the increased expression of FASN in SAT AD subpopulations compared with VAT, the increased expression of ME-markers WT1, UPK3B, and MSLN in VAT compared with SAT, and the decreased expression of CD68 and S100A12 in SAT MAC compared with VAT. Here, S100A12 is linked to Alzheimer disease.