The analysis revealed that age (1.044 [1.031–1.058]; P < 0.001), CHF (2.521 [1.579–3.938]; P < 0.001), previous AIS/TIA (2.165 [1.446–3.192]; P < 0.001), NIHSS score (1.228 [1.184–1.273]; P < 0.001), CRP (1.008 [1.005–1.011]; P < 0.001), BNP (1.016 [1.012–1.020]; P < 0.001) and cortical infarction (2.559 [1.860–3.528]; P < 0.001) were significantly associated with AFDAS in the training cohort (Figure 3). This evidence concerns the gene NPPB and transient ischemic attack.