Comparison of both assays revealed that the log2 fold change of 8 genes which showed upregulation in the mRNAseq assays of the HCC group compared to the control group (Lamc2, Emp1, Scd, Bdkrb2, Slc7a11, Igfbp2, Wnt5a, and Col1a1) were also upregulated in the RT-qPCR assays (Figure 1A). This evidence concerns the gene IGFBP2 and hepatocellular carcinoma.