TLR7 and melanoma: Because thiol-binding mediates intratumoralretention, it should prevent the documented toxicity associated withthe systemic exposure to TLR7 agonists.16,47 We observedthat p(Man-TLR7-PDS) administered subcutaneously in healthy mice andintratumorally in B16F10 melanoma- and CT26 adenocarcinoma-bearingmice did not lead to weight loss (Figures S14–S16).