Here, to demonstrate immune agonizationby the copolymer, we used macrophages as a model, also noting thehigh frequency tumor-associated macrophages expressing macrophagemannose receptor CD206.38,39 We stimulated macrophage-likeRAW 264.7 cells and observed a dose-dependent increase in proinflammatorycytokines that are downstream of TLR7 signaling (Figure 3e–i).40 As a positive control for our assay, we included a well-knownTLR7/8 agonist R848 at our top concentration. The gene discussed is TLR7; the disease is neoplasm.