E-ice-COLD-PCR has been used to detect somatic mutations in BRAF in fresh frozen tissue, FFPE, and plasma samples of melanoma patients 15; mutations in KRAS in fresh frozen tissues of CRC patients 14; and mutations in KRAS in cfDNA of metastatic CRC patients 35, but it has not been evaluated in the early stage of CCR development. The gene discussed is KRAS; the disease is colorectal carcinoma.