Prior to cfDNA analysis, we evaluated the sensitivity of E-ice-COLD-PCR and endpoint PCR followed by next generation sequencing (NGS) to detect mutations in Ctnnb1 and Kras in 5 tumor samples (mice: M1, M3 M8, M10 and M16) previously analyzed with Sanger sequencing. The gene discussed is CTNNB1; the disease is neoplasm.