As one of the active flavonoids, Icariside II can regulate eNOS phosphorylation rapidly which suggests that Icariside II may assist in the treatment of acute diseases (such as myocardial infarction and cerebral ischemia) and erectile dysfunction which has been preliminary explored in other in vivo and in vitro studies (Hu et al., 2020; Gao et al., 2020; Liu et al., 2020; Gu et al., 2021; Xu et al., 2015). Here, NOS3 is linked to brain ischemia.