To determine the role of DNA-PKcs during ZIKV infection, we used CRISPR/Cas9 editing of the PRKDC gene to generate DNA-PKcs-deficient A549 and RPE epithelial cells, referred to as A549PRKDC-/-(Figures S1A, B) and RPEPRKDC-/-(Figure S1C). This evidence concerns the gene PRKDC and Zika virus infectious disease.