Comparing the silicosis group to the controls, the CD25+ percentages of the CD3+ CD4+ subpopulation was significantly elevated at all concentrations of NM-200 (Figure 1D) but only at 10 μg/ml in the presence of Ludox (Figure 1C), although a significant increase was noted when compared to unstimulated population, at all concentrations of Ludox. This evidence concerns the gene CD4 and silicosis.