ACTA2 and hepatocellular carcinoma: Macrophage (cluster 1; markers: CD163, CD68, CD14), T cells (clusters 2, 8; markers: CD2, CD3D, CD3E, and CD3G), B cells (cluster 9; markers: CD19, CD79A, and MS4A1), plasma cells (cluster 4; markers: CD79A and JSRP1), mast cells (cluster 10; markers: TPSAB1 and CPA3), fibroblasts (cluster 7; markers: ACTA2, PDGFRB, and NOTCH3), endothelial cells (cluster 6; markers: PECAM1), and hepatocellular carcinomas (cluster 0, 3, 5; GPC3, CD24, and MDK) were clustered according to immune cell markers (Figure S3).