GSEA enrichment analysis also showed that the regulation of CD8+T cells, CD4+T cells, NK cells and Treg cells was closely related to the expression level of MYCN. In the past few years, more attention has been paid to the research on MYC, there have also been studies focusing on the effect of age of onset on immune cell infiltration in NB (39). The gene discussed is CD8A; the disease is neuroblastoma.