In this study, differentially expressed genes (DEGs) of MYCN positive NB were screened out by GEO and ArrayExpress databases, and gene co-expression network was constructed by WGCNA, Lasso regression and Roc analyses to obtain modules and hub genes that play important biological roles in MYCN positive NB. The immunocyte correlation analyses of MYCN positive NB, MYCN negative NB and hub genes were also carried out. Figure 1 illustrates the workflow chart of data preparation, processing, analysis, and validation. The gene discussed is MYCN; the disease is neuroblastoma.