Next, the APscore as a binary variable entered into Cox regression model, and then was an independent prognostic predictor of aGC patients after adjusting other clinicopathologic characteristics, including Lauren subtype, age, gender, cancer grade, CD274 expression, TMB and the ratio of M1 Macrophages to M2 Macrophages (HR=4.42, 95% CI:1.36−14.28, Figure 3SH). This evidence concerns the gene CD274 and cancer.