Each tumor was cut into a 4 μm section, pretreated in ethylenediaminetetraacetic acid (EDTA) buffer at pH 9.0 for 20 min, and incubated with mouse monoclonal antibodies against BAP-1 at dilution 1:40 (clone C-4; Santa Cruz Biotechnology, Dallas, TX) and a red chromogen secondary antibody kit (Leica Biosystems, Nußloch, Baden-Wurttemberg, Germany), and finally counterstained with hematoxylin and rinsed with deionized water. The gene discussed is BAP1; the disease is neoplasm.