Mechanistically, upon blockade of GLS, HCC cells were shown to alternatively take up glutamine through ASCT2 to generate glutathione which counteracted ROS; therefore, co‐blockade of GLS and ASCT2 induced ROS, limiting HCC growth.[35] Here, we further showed that glutamine deficient diet significantly suppressed HCC in multiple mouse HCC models. Here, GLS is linked to hepatocellular carcinoma.