GLS and hepatocellular carcinoma: Consistent with glutamine depletion, GLS inhibition increased the pyruvate flux through PDH and PC in HCC cells (EV) (Figure 5A–D), whereas KO of PDHA, PDHB, or PC decelerated the flux through PDH, or PC as indicated by the reduced [M+2] or [M+3] labelled α‐KG and succinate (Figure 5B–D).