BPTES, the GLS inhibitor, has been widely applied to suppress the conversion of glutamine to glutamate in cancer study.[25, 26] Interestingly, we found the combined treatment of PDH or PC inhibitor and BPTES most significantly suppressed the proliferation of HCC cells than single treatments of PDH/PC inhibitor or GLS inhibitor. This evidence concerns the gene PDP1 and hepatocellular carcinoma.