Finally, the effects of the eL31-DEPDC1 axis on CRC cell behaviors were investigated, we constructed eL31-overexpressed, DEPDC1-silenced as well as DEPDC1-silenced combining with eL31-overexpressed HCT 116 cell models to perform recovery experiments (Additional file 1: Figure S3). The gene discussed is RPL31; the disease is colorectal carcinoma.