As MHC-II-CD4 and CD40L-CD40 interactions were found to be enriched in the CaMKK2 KO immune TME via cell–cell communication analysis, and as we observed improved tumor penetrance of CD4+ T cells, we utilized confocal microscopy to determine if there were increased myeloid-CD4+ interactions in CaMKK2 KO mice. The gene discussed is CD4; the disease is neoplasm.