In GBM patients, the expression of ANXA1, CALR, CXCL10, FPR1, HGF, IFNAR1, MET, P2RY2, TLR3, and TLR4 were significantly upregulated in GBM1 immune subtype, while EIFF2AK3 was significantly upregulated in GBM2 (Fig. 6B). The gene discussed is TLR4; the disease is glioblastoma.