In this study, we discovered changes in the relative frequencies of distinct alternative splicing isoforms in gastric cancer cells significantly associated with the expression level of ESRP1. We found the two isoforms of LRRFIP2, whose expressions heavily depended on the expression of ESRP1 and determined the metastatic fate of gastric cancer cells through differential protein-protein interaction with a methyltransferase protein, CARM1. Here, ESRP1 is linked to gastric cancer.