NUPR1 and liver cancer: To demonstrate the role of NUPR1 in the growth and migration of liver cancer cells, we overexpressed NUPR1 in MHCC-97H and SK-Hep1 cell lines and stably knocked down NUPR1 in Huh7 and SMMC-7721 cell lines based on their expression of endogenous NUPR1(Supplementary Fig. 1A) [14].