Our results showed that Ets1 directly interacts with Sp1 independent of DNA in SW480 colon cancer cells, and knockdown ofSp1 caused a decrease in the expression level of Ets1 protein, in agreement with a previous study that showed that in MC3T3-E1 cells, the knockdown ofSp1 reduced the expression of Ets1 at the transcriptional level[38]. The gene discussed is SP1; the disease is malignant colon neoplasm.