To validate neuron and glioma synaptic interactions were involved in GBM location predilection, we analyzed the spatial correlation of tumor distribution with positron emission tomography‐derived average neurotransmitter maps, covering GABAergic (GABAa), dopaminergic (D2, DAT), serotonergic (5HT1a, SERT) and noradrenergic (NAT) systems. This evidence concerns the gene SLC6A3 and glioma.