Interestingly, NECTIN2–TIGIT interaction was enriched in proliferating TAMs and CD4_C3_CXCL13, CD4_C3_FOXP3 and CD8_C2_GNLY cell sub‐clusters, indicating that Proli_TAM may lead to T cell exhaustion in osteosarcoma PT tissues. Here, CD8A is linked to osteosarcoma.