We found that missense mutation of LZTR1 was the main type of genetic alteration and X217_splice alteration in the Kelch-3 domain, which was detected in 9 cases of liver hepatocellular carcinoma, 3 cases of mixed germ cell tumor, 2 cases of embryonal carcinoma, 1 case of kidney renal papillary cell carcinoma, 1 case of seminoma, and 1 case of mucinous carcinoma. This evidence concerns the gene LZTR1 and seminoma.