These data were confirmed by coculturing human Notch-dependent T-ALL cell lines and healthy donor derived PB mononuclear cells in vitro, resulting in increased CD14+HLA-DRlow/neg MDSC accumulation and T-cell suppression; effects that were not observed with T-ALL cells that did not express Notch1- or Notch3-activated protein (79). This evidence concerns the gene NOTCH3 and acute lymphoblastic leukemia.