Notably, the presence of a differentially expressed non-annotated exon between exon 12 and 13 in SPIRE1, which we detected in cancer cell lines (Figure 7B), was also identified in BRCA, LUAD, KIPAN, PRAD, and THCA cohorts by DJEC DB data using gene-wise splicing visualization (Figure 9). The gene discussed is SPIRE1; the disease is cancer.