Although yeaX and CntA showed a reduced tendency, we found that the enzymatic genes for the biosynthesis of trimethylamine (TMA) were non-discriminatory between individuals with NGT and patients with T2D, as shown by CutD, CutC, TorA, CntB, GrdH, yeaX, and CntA in Supplementary Figures S2A,B. Here, CUTC is linked to type 2 diabetes mellitus.