MAGE family member B1 (MAGEB1) and PRKCZ were highly expressed, and four antigens, G antigen 1 (GAGE1), rhinophilin-associated tail protein 1 (ROPN1), sperm protein associated with the nucleus, X-linked, family member A1 (SPANXA1) and protein kinase C zeta (PRKCZ), had higher autoantibody titres in PCa serum than in BPH serum. The gene discussed is MAGEB1; the disease is benign prostatic hyperplasia.