In 2019, the same research team performed Single-cell sequencing of CD45+ immune cells selected from HCC tumors, liver lymph nodes (LN), blood and ascites, compared the two methods of SMART-seq and 10x Genomics, revealed that LAMP3+ DCs were mature DCs from tumor to local LN and play a major role in T-cell activation; different macrophage subpopulations exist in tumors, among which tumor-associated macrophages (TAM) are associated with the survival prognosis of HCC patients (Zhang et al., 2019b). Here, LAMP3 is linked to hepatocellular carcinoma.