In 2017, The Center for Frontier Innovation in Biomedicine at Peking University selected T cells in HCC patients for deep single cell RNA sequencing, identified 11 T cell subsets based on their molecular and functional properties and delineated their developmental trajectories, and the genes characteristics of each subset were finally identified; And found that layilin was upregulated in activated CD8+ T cells and Tregs, and inhibited the function of CD8+ T cells in vitro (Zheng et al., 2017). Here, CD8A is linked to hepatocellular carcinoma.