Single cell transcriptome analysis for HCC CD45+ immune cells. SMART-seq is able to distinguish closely related cell subpopulations, while 10x Genomics can be used to analyze rare cell populations with low cell numbers. LAMP3+ DCs are mature DCs from the tumor to the local LN and play a major role in T-cell activation. Different macrophage subpopulations exist in tumors, among which tumor-associated macrophages (TAM) are closely associated with survival prognosis. Here, LAMP3 is linked to hepatocellular carcinoma.