Single cell analysis against HCC T cells. Different subpopulations of T cells can be detected in the tumor microenvironment. Trajectory analysis revealed a transition from naive T cells to depleted or cytotoxic T cells.TCR sequencing revealed the distribution of T cells in the tumor. LAYN as a suppressor marker gene for depleted CD8+ T cells and Treg expression. This evidence concerns the gene LAYN and neoplasm.