When the concentration of cisplatin was 1, 2, and 4 μg/ml, cell viability of HS683/DDP and U87/DDP with silencing NR5A2 was notably lower than that of the si‐NC group, showing that silencing NR5A2 noticeably increased chemosensitivity of glioma cells to cisplatin (p < 0.05). Here, NR5A2 is linked to central nervous system cancer.