CD4 and Anxiety: Multivariate ANOVA revealed that anxiety and the symptom response to the nutrient challenge were both independently associated with FGID, anxiety, nutrient challenge, and the first genus factor all independently discriminated FGID vs controls, whereas gut homing CD4+ α4+ β7+ CCR9+ and CD8 cells were independently associated with SR-NCWS (Table 5 and Table 6).