In this work, obvious CAV1 overexpression was observed in the liver after i.v. AAV2/8CAV1 treatment or in both the liver and the gallbladder through i.p. AAV2/8CAV1 injection allowed us to distinguish the role of CAV1 in gallbladder motility and its contribution to the prevention of CGD, independent of changes in bile cholesterol saturation. This evidence concerns the gene CAV1 and chronic granulomatous disease.