Previous work by Tharp et al. [34] using CGD mice found that maintaining gallbladder physiological function via gallbladder long-chain fatty acid transporter 2 (FATP2) deletion by systemic i.v. injection of AAV-delivered short hairpin RNA (shRNA) is sufficient to prevent gallstone formation, even in the presence of supersaturated bile cholesterol. The gene discussed is SLC27A2; the disease is gallstones.