Given the vital role of UHRF1 in DNA methylation, we performed bisulfite sequencing of MXD4 to test the DNA methylation level of MXD4 and found that knocking down UHRF1 decreased the level of MXD4 DNA methylation at the CpG sites around the TSS in AML cells (Fig. 5c, d). This evidence concerns the gene MXD4 and acute myeloid leukemia.