For CD44, a well-known CSC marker regulating cancer stemness in various cancer types32,33, there is some discrepancy between the expression levels of mRNA and protein; the transcript levels of CD44 were high only in SP1030 cells compared to mother cells (Supplementary Table 1 and Supplementary Fig. 5a), but, in image analysis for protein expression from confocal images, the CD44 density of ROIs increased in SP0926 (mean: 19.3) and SP1030 cells (mean: 24.1) compared to mother Panc0203 cells (mean: 15.3; Supplementary Fig. 5b). The gene discussed is CD44; the disease is cancer.