Three HLA class I-expressing hepatocellular carcinoma (HCC) cell lines (HLE, SMMC-7721, and HepG2; Supplementary Table 3) were transduced with an expression construct that allowed the Doxycycline (Dox)-dependent expression of the DNAJB1-PRKACA fusion protein (Fig. 3a, b, Supplementary Fig. S3a). Here, PRKACA is linked to hepatocellular carcinoma.