In this study, we novelty generated a whey-derived antioxidative peptide (YVEEL) (17) conjugated with a powerful cell-penetrating peptide TAT (18) [Tat (48–60) YVEEL], and investigated for the first time the role of Tat (48–60) YVEEL in the protection against experimental NEC both in vitro and in vivo. This evidence concerns the gene TAT and necrotizing enterocolitis.