To determine the biological functions of TOP2A in HCC cells, we initially detected the expression of TOP2A in L02 and 8 HCC cell lines (MHCC97L, MHCC97H, HCCLM3, SMMC7721, BEL-7404, HepG2, SK-HEP-1, and Huh7). The gene discussed is TOP2A; the disease is hepatocellular carcinoma.