To further elucidate the role of EVs in ADPKD development in the distal segments of the nephron, we evaluated the EV release in wild type and Pkd1 knockout (Pkd1-/-) mouse distal convoluted tubule (mDCT15) and mIMCD3 cells, as well in an in vivo murine model of ADPKD. The gene discussed is PKD1; the disease is autosomal dominant polycystic kidney disease.