ATG5, ATG7, and BECLIN1 were targeted for 48 h prior to infection (MOI 0.001 and 0.1), and the influence of the silencing on the capacity of Huh7 cells to produce infectious DUGV particles was assessed by running a plaque assay on VeroE6 cells with dilutions of culture supernatants that were collected 48 h post-infection. The gene discussed is BECN1; the disease is infection.