MIF and infection: To evaluate the efficiency of this process in the presence of MIF, infection percentages were evaluated in CD4TLs after five days of co-culture by p24 antigen intracellular staining and flow cytometry analysis (Figure 3A), by evaluating the concentration of p24 antigen in culture supernatants (Figure 3B), and by quantitation of proviral DNA by real-time PCR (Figure 3C).