NUCLEOLIN and neoplasm: Our work demonstrates that: (i) Gq-DNA transfection is a low cytotoxic and efficient strategy for gene delivery; (ii) Gq-DNA transfection selectively deliver DNA into tumor cells with high-level nucleolin; (iii) Gq-DNA transfection work independently of cell division by directly delivering DNA into nuclei; (iv) Gq-DNA transfection is characterized by ease of synthesis and low cost (Cost Accounting is in the Supplementary Materials).