Triple-gene therapy in the acute phase and for the preventive treatment of stroke in a mini-pig model, using the approach of the intravenous infusion of the autologous genetically enriched leucoconcentrate producing recombinant neuroprotective molecules (VEGF, GDNF and CAN), demonstrated beneficial effects on the preservation and recovery of the brain after ischemic stroke. Here, GDNF is linked to stroke disorder.