The upregulation of NEO1 in adipocytes after the treatment with NTN-1 together with the lack of changes in the expression of UNC5B and DCC suggest that NTN-1 mainly signals through NEO-1 in VAT during obesity, being reinforced by the positive correlation found between the expression levels of NTN1 and NEO1 in the VAT. This evidence concerns the gene NTN1 and obesity due to melanocortin 4 receptor deficiency.